| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Cloacibacillus evryensis | ATTTCCTGACGCTGCGCT | TACGGCTGGCATTTCTCCC | 60.05 | 59.78 | 157 | 97 |
100.00%
|
100.00%
|
| Primer 22 | Cloacibacillus evryensis | ATTTCCTGACGCTGCGCT | ATACCCTCCTGCGTGAACC | 60.05 | 59.09 | 280 | 97 |
100.00%
|
100.00%
|
| Primer 23 | Cloacibacillus evryensis | CGCTGCAGAGGGAGTTCAT | TTCGCCTGCCTTTCGAGT | 59.78 | 59.26 | 217 | 97 |
100.00%
|
100.00%
|
| Primer 24 | Cloacibacillus evryensis | TCGGCTTTGTCAACCGCT | CGCTGAAGGCCTGGATCAA | 59.89 | 60.08 | 282 | 97 |
100.00%
|
100.00%
|
| Primer 25 | Cloacibacillus evryensis | TCGGCTTTGTCAACCGCT | ATCCCGTCGTTCAGCATGG | 59.89 | 60.15 | 187 | 97 |
100.00%
|
100.00%
|
| Primer 26 | Cloacibacillus evryensis | TCATCGTGGACGCCTCAA | ATATGCGTGCCAGGCGAA | 58.63 | 59.81 | 239 | 97 |
100.00%
|
100.00%
|
| Primer 27 | Cloacibacillus evryensis | ACGCAAACGATCTCGGCT | GGCTTGATGTCCGTGCAGA | 59.74 | 60.38 | 196 | 94 |
100.00%
|
100.00%
|
| Primer 28 | Cloacibacillus evryensis | CGCTGGAACCCGAAGATGA | TGGCGTTTGGTTCCCACA | 59.78 | 59.72 | 161 | 94 |
100.00%
|
100.00%
|
| Primer 29 | Cloacibacillus evryensis | TGCACCGATTTCGCCTGT | TTGCCAGCACGTTGAGGA | 59.97 | 59.49 | 151 | 94 |
100.00%
|
100.00%
|
| Primer 30 | Cloacibacillus evryensis | TGCACCGATTTCGCCTGT | TCAGGCCTGATCTCACGGA | 59.97 | 60.00 | 281 | 94 |
100.00%
|
100.00%
|